ࡱ > s r W (
/ 0 D A r i a l n g s b b / b hb 0 b z[ 0xb D T a h o m a g s b b / b hb 0 b z[ 0xb " D W i n g d i n g s b b / b hb 0 b z[ 0xb @ .
@ n ? " d d @ @@ `` 0 ( P ? %
*
. 0 A A ` f $ @ ' ʚ; ʚ; g 4 B d B d b z[ 0 p b p p p @ < 4 d d d d Ķb 0 b . b < 4 B d B d Ķb < 0 b b 0 _ _ _ P P T 1 0
_ _ _ P P T 9
?
% HAEMOGLOBIN ESTIMATION ( S a h l i s m e t h o d Aim T o e s t i m a t e t h e H a e m o g l o b i n l e v e l s i n m g / d l u s i n g S a h l i s m e t h o d 2 Equipment S a h l i s h a e m o g l o b i n o m e t e r w i t h t u b e , p i p e t t e , c o l o u r c o m p a r a t o r a n d s t i r r e r
H y d r o c h l o r i c a c i d , 0 . 1 N
D i s t i l l e d w a t e r
B l o o d s a m p l e $ h 2 S A H L I S H A E M O G L O B I N O M E T E R (
PROCEDURE F i l l N / 1 0 H C l u p t o m a r k 2 o f t h e t u b e
P i p e t t e o u t 2 0 L o f b l o o d , w i p e t i p w i t h c o t t o n t o r e m o v e e x c e s s b l o o d
I n t r o d u c e b l o o d i n t o H C l i n t h e t u b e
M i x , k e e p f o r 1 0 m i n u t e s
A d d d i s t i l l e d w a t e r , s t i r , c o m p a r e c o l o u r i n c o m p a r a t o r
C o n t i n u e a d d i n g w a t e r a n d m i x i n g u n t i l c o l o u r m a t c h e s
R e a d l o w e r m e n i s c u s o n t u b e f o r H b i n g / d L
8 F Z Z F t
) M ' Fill N/10 HCl up to mark 2 of the tube ( ( $
6 P i p e t t e o u t 2 0 L o f b l o o d " (
( 4
Introduce blood into tube
Mix, keep for 10 minutes Add distilled water, stir
, Read Hb from lower meniscus, express as g/dl - - ( &
QUESTIONS Describe the procedure
What is the principle behind the method?
What are the advantages & the disadvantages of this method?
What other methods are available?
Which method is considered ideal?
What is the reference haemoglobin range?
What is the importance of haemoglobin estimation?
* " " , "
MORE RELATED QUESTIONS D e f i n e a n e m i a / p o l y c y t h e m i a
C l a s s i f y a n e m i a .
N a m e s o m e c a u s e s f o r a n e m i a / p o l y c y t h e m i a
W h a t i s D r a b k i n s s o l u t i o n ?
_ _ _ _ _ _ _ _ _ _ _ _ < u "
u > 0 b ` 33 ` S f 3 f ` 33 g ` f ` www 3 PP ` ZX db mo ` \ ғ 3 y` Ӣ ` 3f 3f f ` 3f 3 FK f ` hk] www f ܹ ` ff >>\ `Y{ ff ` R>& - {p _/ ̴ > ? " d d @ , | ? " d d @ " @ ` n ? " d d @ @@ `` P R @ ` ` p > > f (
6 $ `}
T Click to edit Master title style !
!
0 `
R Click to edit Master text styles
Second level
Third level
Fourth level
Fifth level !
S
0 h ^ `
> *
0 , ^
@ *
0 ^ `
@ * B
s * h ? 33 8 0 _ _ _ P P T 1 0 . $v D e f a u l t D e s i g n b
K0 P (
r
S d#I > I
S L$I `
< $ D
0 I
H
0 h ? 33 _ _ _ P P T 1 0 . $v + ' D ' b =
@B DW ' =
@B A ? %, ( < + O %, (
< + D ' b = % ( % ( D ' = % ( D ' =4 @B B B B % ( D ' = 1 : B v i s i b l e *o 3 >+ B# s t y l e . v i s i b i l i t y < * % ( D ' = + 4 8 ?\ C B # p p t _ x B C B # p p t _ x B *Y 3 > B
p p t _ x < * D ' = + 4 8 ?d C B 1 + # p p t _ h / 2 B C B # p p t _ y B *Y 3 > B
p p t _ y < * +
b K0 0 $ (
r
S mI `}
I
r
S nI ` I
H
0 h ? 33 _ _ _ P P T 1 0 i . $ 4 + D= ' b =
@B + }
b @ $ (
r
S rI `}
I
r
S sI ` I
H
0 h ? 33 _ _ _ P P T 1 0 i . %& + D= ' b =
@B +
b P 1 > : (
r
S dyI `}
I
l
6 " `
~ r
H v d @ " ` r
@
N h* G` v @ " `
`
s * " `
` 2
s * " ` @ p `
s * " ` p
l b
6 " ` `Ppp f r
0 Z " ` @ ~
H v 2 @ " ` d ~
H v 2 @ " `
# l ? v 2 @ ? "6 ? @ ` N N N ? N d
Z V\^ y2 " `
# l ? v 2 @ ? "6 ? @ ` N N N ? N p ~ r
H v 2 @ " ` p R B
s * D > d R B
s * D >
R B
s * D >
R B
s * D
< I t
2 0 l m a r k ,
< I
Comparator
!
< XI 0
G Haemoglobinometer pipette R B
"
s * D p
#
< Dw
D Haemoglobinometer tube B
$
# l ? v 2 @ ? "6 ? @ ` N N N ? N @
%
< I
Stirrer r
&
# l Z ? v @ ? "6 ? @ ` N N N ? N
( R B
'
s * D
L B
(
c $ D L B
)
c $ D L B
*
c $ D @ @ L B
+
c $ D L B
,
c $ D L B
-
c $ D ` ` L B
.
c $ D L B
/
c $ D
L B
0
c $ D
L B
1
c $ D
L B
2
c $ D @ @ L B
3
c $ D L B
4
c $ D R B
7
s * D
8
< I
Mark 2 ` B
9
s * " `
B
:
B ? ? "6 ? @ ` N N N ? N p
~
;
H v 2 @ " ``P
~
<
H v 2 @ " `P
`
=
s * " ` 0 H
0 h ? 33 _ _ _ P P T 1 0 i . & [ + D= ' b =
@B + }
b ` $ (
r
S I `}
I
r
S tI I
H
0 h ? 33 _ _ _ P P T 1 0 i . ) + D= ' b =
@B +
b p $ ^ (
$ r
$ S ثI =
I
r
$
# l Z ? v @ ? "6 ? @ ` N N N ? N
0 L B
$
c $ D L B
$
c $ D L B
$
c $ D H H L B
$
c $ D L B
$
c $ D
L B
$
c $ D h
h
L B
$
c $ D
L B
$
c $ D ( ( L B
$
c $ D L B
$
c $ D L B
$
c $ D H H L B
$
c $ D L B
$
c $ D
R B
$
s * D
$
< I `
G
Mark 2
$
0 {T " `
0 , $ D
0 R B
$
s * D p P
(
$
< xI
<g
> N/10 HCl H
$ 0 h ? 33 e ] _ _ _ P P T 1 0 = . a + = D ' b =
@B D ' =
@B A ? %, ( < + O %, (
< + D ' b = % ( % ( D ' = % ( D3 ' =4 @B B B B % ( D ' = 1 : B v i s i b l e *o 3 >+ B# s t y l e . v i s i b i l i t y < * $ % ( D ' = -o 6 B d i s s o l v e *< 3 < * $ +
b ) ! ( (
( r
( S I `
I
~
(
H v 2 @ " ` 4
~
(
H v 2 @ " `
(
# l ? v 2 @ ? "6 ? @ ` N N N ? N 4
(
Z V\^ y2 " `
(
# l ? v 2 @ ? "6 ? @ ` N N N ? N D
p ~ r
(
H v 2 @ " ` p D
R B
(
s * D > 4
R B
(
s * D
(
< I 0 D
2 0 L m a r k , B
(
s * D Ԕ P
P
, $ D
0
(
0 I " `
( R B
( @
s * D
(
0 I
= ) Wipe excess blood
around tip with cotton f
(
0 " ` ` x
(
B 2 " ` "
(
f ? 2 ? "6 ? @ ` N N N ? N p` ~ "
(
H v 2 @ "
` "
(
s * 3f " ` P
(
< @I
Blood sample H
( 0 h ? ( 33 _ _ _ P P T 1 0 m . @q + DA ' b =
@B D ' =
@B A ? %, ( < + O %, (
< + D3 ' b = % ( % ( D ' = % ( Dc ' =4 @B B * B B % ( D ' = 1 : B v i s i b l e *o 3 >+ B# s t y l e . v i s i b i l i t y < * ( % ( D ' = -g 6 B f a d e *< 3 < * ( D ' = + 4 8 ?\ C B # p p t _ x B C B # p p t _ x B *Y 3 > B
p p t _ x < * ( D ' = + 4 8 ?b C B # p p t _ y + . 1 B C B # p p t _ y B *Y 3 > B
p p t _ y < * ( +
b $, H (
, r
, S I `}
I
r
,
# l Z ? v @ ? "6 ? @ ` N N N ? N 8 L B
,
c $ D 0 L B
,
c $ D 8
0 8
L B
,
c $ D
0
L B
,
c $ D
0
L B
,
c $ D X 0 X L B
,
c $ D 0 L B
,
c $ D 0 L B
,
c $ D x 0 x L B
,
c $ D 0 L B
,
c $ D 8
0 8
L B
,
c $ D
0
L B
,
c $ D
0
L B
,
c $ D X0 X f
,
0 {T " `
0 R B
,
s * D ` p x
,
< I \
> N/10 HCl ~
,
H v 2 @ " ` `
,
# l ? v 2 @ ? "6 ? @ ` N N N ? N
` R B
,
s * D >
`
R B
,
s * D Ԕ
~
,
H v 2 @ " `
,
3 r 5 ? v 2 @ ? "6 ? @ ` N N N ? N
p
r
,
N v 2 @ " ` 0
H
, 0 h ? 33 _ _ _ P P T 1 0 . @ + D ' b =
@B DM ' =
@B A ? %, ( < + O %, (
< + D ' b = % ( % ( D ' = % ( D ' =4 @B B % B B % ( D ' = -g 6 B f a d e *< 3 < * , D
' = + 4 8 ?X C B
p p t _ x B C B
p p t _ x B *Y 3 > B
p p t _ x < * , D% ' = + 4 8 ?` C B
p p t _ y B C B p p t _ y - . 0 3 B *Y 3 > B
p p t _ y < * , ) ? DA ' = + 4 8 ?\ C B
p p t _ y B C B p p t _ y + 1 B *Y 3 > B
p p t _ y < * , % ( ) ? D ' = 1 : B h i d d e n *o 3 >+ B# s t y l e . v i s i b i l i t y < * , % ( + 7
b z r )0
(
0 r
0 S I `}
I
0 6 I " ` ` I
r
0
# l Z ? v @ ? "6 ? @ ` N N N ? N
0 L B
0
c $ D L B
0
c $ D L B
0
c $ D H H L B
0
c $ D L B
0
c $ D
L B
0
c $ D h
h
L B
0
c $ D
L B
0
c $ D ( ( L B
0
c $ D L B
0
c $ D L B
0
c $ D H H L B
!0
c $ D L B
"0
c $ D
(0
0 L " `
, $ D
0 H
0 0 h ? 33 e ] _ _ _ P P T 1 0 = . . + p| D ' b =
@B D ' =
@B A ? %, ( < + O %, (
< + D ' b = % ( % ( D ' = % ( D3 ' =4 @B B B B % ( D ' = 1 : B v i s i b l e *o 3 >+ B# s t y l e . v i s i b i l i t y < * (0 % ( D ' = -o 6 B d i s s o l v e *< 3 < * (0 + +
b n
f
4 (
4 r
4 S ,)R `}
R
l
4
6 " ` P
0 ~ r
4
H v d @ " ` 0 r
4 @
N h* G` v @ " ` /O
, `
4
s * " ` P
0 ` 2
4
s * " ` P
`
4
s * " ` l b
4
6 " `
f r
4
0 Z " `
p ( R B
4
s * D > 0 R B
4
s * D > P
P
0 ` B
4
s * " ` B
4
B ? ? "6 ? @ ` N N N ? N P
~
4
H v 2 @ " @ ~
4
H v 2 @ "
`
4
s * " ` P
B
4
# l ? v 2 @ ? "6 ? @ ` N N N ? N
B
4
# l ? v 2 @ ? "6 ? @ ` N N N ? N `
r
4
N Z $ d " `
p ( , $ D
0 H
4 0 h ? 33 e ] _ _ _ P P T 1 0 = . W2 + U( D ' b =
@B D ' =
@B A ? %, ( < + O %, (
< + D ' b = % ( % ( D ' = % ( D3 ' =4 @B B B B % ( D ' = 1 : B v i s i b l e *o 3 >+ B# s t y l e . v i s i b i l i t y < * 4 % ( D ' = -o 6 B d i s s o l v e *< 3 < * 4 +
b 8 (
8
8
6 hI `}
l < Continue adding, stirring until colour matches with standard = = $ l
8
6 " ` PP
` ~ r
8
H v d @ " ` ` P r
8 @
N h* G` v @ " ` _O
\ `
8
s * " ` P
` ` 2
8
s * " ` P
`
8
s * " ` P l b
8
6 " `
f r
8
0 Z " ` P
p X R B
8
s * D > P` R B
8
s * D > PP
P
` ` B
8
s * " ` P B
8
B ? ? "6 ? @ ` N N N ? N P P
~
8
H v 2 @ " @ ~
8
H v 2 @ "
`
8
s * " ` PP
r
8
H Z $ v @ " `
p X , $ D
0 H
8 0 h ? 33 ] U _ _ _ P P T 1 0 5 . X o + t D ' b =
@B D ' =
@B A ? %, ( < + O %, (
< + D ' b = % ( % ( D ' = % ( D+ ' =4 @B B
B B % ( D ' = 1 : B v i s i b l e *o 3 >+ B# s t y l e . v i s i b i l i t y < * 8 % ( D ' = -g 6 B f a d e *< 3 < * 8 +
b -< ` (
< r
< S XR `}
R
r
<
# l Z ? v @ ? "6 ? @ ` N N N ? N 0 0 L B
<
c $ D p L B
<
c $ D p L B
<
c $ D H p H L B
<
c $ D p L B
<
c $ D
p
L B
<
c $ D h
p h
~ r
*<
H Z $ v @ " `
0 L B
<
c $ D
p
L B
!<
c $ D ( p ( L B
"<
c $ D p L B
#<
c $ D p L B
$<
c $ D H p H L B
%<
c $ D p L B
&<
c $ D
p
R B
+<
s * D
` 0
,<
< `R
D Read Hb level H
< 0 h ? 33 _ _ _ P P T 1 0 i . Y`- + D= ' b =
@B +
b @ : (
@ r
@ S hR `}
R
@ S l߅ ` R
" p`Pp H
@ 0 h ? 33 _ _ _ P P T 1 0 i . Z`ˉ + D= ' b =
@B +
b D : (
D r
D S ԁR `}
R
D S 4R ` R
" p`Pp H
D 0 h ? 33 _ _ _ P P T 1 0 i . Z p\ + D= ' b =
@B + rD _ -& ( O+ C oF T g { ] a " ?
X
Oh +'0 ` h
HAEMOGLOBIN ESTIMATION V I M S - 4 V I M S - 4 50 Microsoft PowerPoint N @ xD @ }wV&@ .! G g R ( y - @ ! x - - ' . @ Arial - ( 2
B
HAEMOGLOBIN ESTIMATION . Courier New ( (r>3Xb R - - . @ Arial - 2
_ ) Sahlis . - . @ Arial - 2
_ : method . -
՜. +,0
On-screen Show Vydehi e Ȳ 6 A
Arial Tahoma
Wingdings Default Design HAEMOGLOBIN ESTIMATION Aim
Equipment SAHLI’S HAEMOGLOBINOMETER
PROCEDURE ( Fill N/10 HCl up to mark 2 of the tube Pipette out 20 μL of blood Introduce blood into tube Mix, keep for 10 minutes Add distilled water, stir Slide 11 - Read Hb from lower meniscus, express as g/dl
QUESTIONS MORE RELATED QUESTIONS Fonts Used Design Template
Slide Titles # _㤲 V I M S - 4 V I M S - 4
! " # $ % & ' ( ) * + , - . / 0 1 2 3 4 5 6 7 8 9 : ; < = > ? @ A B C D E F G H I J K L M N O P Q R S T U V W X Y [ \ ] ^ _ ` a c d e f g h i k l m n o p q t R o o t E n t r y dO ) C u r r e n t U s e r j S u m m a r y I n f o r m a t i o n ( Z P o w e r P o i n t D o c u m e n t ( Ȳ D o c u m e n t S u m m a r y I n f o r m a t i o n 8 b